Comparison of a Commercial Multiplex Real-Time PCR to the Cell Cytotoxicity Neutralization Assay for Diagnosis of Clostridium difficile Infections

被引：66

作者：

Huang, Haihui ^{[1
,2
]}

Weintraub, Andrej ^{[1
]}

Fang, Hong ^{[1
]}

Nord, Carl Erik ^{[1
]}

机构：

[1] Karolinska Univ Hosp Huddinge, Div Clin Microbiol, Dept Lab Med, Karolinska Inst, SE-14186 Stockholm, Sweden

[2] Fudan Univ, Inst Antibiot, Huashan Hosp, Shanghai 200040, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 11期

关键词：

TOXIN DETECTION KITS; LABORATORY DETECTION; RAPID DETECTION; FECAL SAMPLES; ALGORITHM; DIARRHEA; CULTURE; GENE;

D O I：

10.1128/JCM.01280-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A commercial multiplex real-time PCR assay (Cepheid Xpert C. difficile assay) for the diagnosis of Clostridium difficile infection was evaluated. The sensitivity and specificity of the Cepheid assay were 97.1% and 93.0% for fresh stools, using the cell cytotoxicity neutralization assay as the reference. Using PCR ribotyping as the reference for ribotype 027 strains, the corresponding figures were 100% and 98.1%, respectively.

引用

页码：3729 / 3731

页数：3

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