Ultrasensitive densitometry detection of cytokines with nanoparticle-modified aptamers

被引：37

作者：

Li, Yuan-Yuan ^{[1
]}

Zhang, Chi ^{[1
]}

Li, Bo-Sheng ^{[1
]}

Zhao, Li-Fan ^{[1
]}

Li, Xiao-Bo ^{[1
]}

Yang, Wen-Jie ^{[1
]}

Xu, Shun-Qing ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, MOE Key Lab Environm & Hlth, Wuhan 430030, Peoples R China

来源：

CLINICAL CHEMISTRY | 2007年 / 53卷 / 06期

关键词：

D O I：

10.1373/clinchem.2006.082271

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Aptamers mimic properties of antibodies and sometimes turn out to be even better than antibodies as reagents for assays. We describe the establishment of an ultrasensitive densitometry method for cytokine detection by nanoparticle (NP)-modified aptamers. Methods: The assay simultaneously uses a gold NP-modified aptamer and a biotin-modified aptamer to bind to the target protein, forming a sandwich complex. The absorbance signal generated by the aptamer-protein complex is amplified and detected with a microplate reader. Results: The assay for platelet-derived growth factor B-chain homodimer (PDGF-BB) was linear from I fmol/L to 100 pmol/L (R-2 = 0.9869). The analytical detection limit was 83 amol/L. The intraassay and interassay imprecision (CVs) was <= 7.5%. Serum concentrations of PDGF-BB determined with the gold NP-modified aptamer assay and with ELISA were not significantly different. Conclusions: The gold NP-modified aptamer assay provides a fast, convenient method for cytokine detection and improves the detection range and the detection limit compared with ELISA. (C) 2007 American Association for Clinical Chemistry.

引用

页码：1061 / 1066

页数：6

共 39 条

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