Discrimination among the clinical isolates of Candida albicans by amplification of the repetitive sequences, alts

被引：3

作者：

Doi, M ^{[1
]}

Chibana, H ^{[1
]}

Nakagawa, Y ^{[1
]}

Tanaka, K ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Dis Mechanism & Control Res Inst, Med Mycol Lab, Nagoya, Aichi 466, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1998年 / 42卷 / 03期

关键词：

PCR; Candida albicans; repetitive sequence;

D O I：

10.1111/j.1348-0421.1998.tb02275.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A primer pair, PB and BSH, which amplified alts, a portion of Candida albicans-specific repetitive sequence, RPS, gave stable and reproducible fingerprint patterns of the strains by polymerase chain reaction (PCR). We applied this method to clinical isolates of C. albicans for strain discrimination. Using PCR fingerprint patterns, we could analyze the relatedness of C. albicans strains including those isolated from children with leukemia and their bedside parents. The results indicated that PCR analysis targeting an alt region gives rise to the same conclusion as the previous study obtained by SmaI RFLP analysis.

引用

页码：227 / 230

页数：4

共 14 条

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