Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry

被引：67

作者：

Guindulain, T

Comas, J

VivesRego, J

机构：

[1] UNIV BARCELONA,DEPT MICROBIOL,E-08028 BARCELONA,SPAIN

[2] UNIV BARCELONA,DEPT SERVEIS CIENTIF TECNICS,E-08028 BARCELONA,SPAIN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 11期

关键词：

D O I：

10.1128/AEM.63.11.4608-4611.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the histograms obtained after DNase and RNase treatments.

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页码：4608 / 4611

页数：4

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