Enhanced production of recombinant B-domain deleted factor VIII from Chinese hamster ovary cells by propionic and butyric acids

被引：22

作者：

Chun, BH

Park, SY

Chung, NY

Bang, WG

机构：

[1] Korea Univ, Coll Life & Environm Sci, Dept Agr Chem, Seoul 136075, South Korea

[2] Green Cross Co, Keeheungeup 449900, Yongin, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 04期

关键词：

alkanoic acid; factor VIII; protein-free medium; sodium butyrate; sodium propionate;

D O I：

10.1023/A:1022301528118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sodium propionate, as well as sodium butyrate, enhanced the production of recombinant B-domain-deleted, factor VIII (rFVIIIdB) by Chinese hamster ovary cells growing in a spinner-flask with a protein-free medium by more than six-fold. The two acids, however, had different cytotoxicities.

引用

收藏

页码：315 / 319

页数：5

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