Comparison of several extraction techniques for multiclass analysis of veterinary drugs in eggs using ultra-high pressure liquid chromatography-tandem mass spectrometry

被引:135
作者
Garrido Frenich, Antonia [1 ]
del Mar Aguilera-Luiz, Maria [1 ]
Martinez Vidal, Jose Luis [1 ]
Romero-Gonzalez, Roberto [1 ]
机构
[1] Almeria Univ, Dept Analyt Chem, Grp Analyt Chem Contaminants, E-04071 Almeria, Spain
关键词
Ultra-high pressure liquid chromatography; Extraction method; Multiclass analysis; Veterinary drugs; Eggs; MACROLIDE ANTIBIOTIC-RESIDUES; LINKED-IMMUNOSORBENT-ASSAY; SOLID-PHASE EXTRACTION; DECISION; 657/2002/EC; ANIMAL-TISSUES; LAYING HENS; PERFORMANCE; MILK; FLUOROQUINOLONE; VALIDATION;
D O I
10.1016/j.aca.2009.12.016
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This study compared four extraction methods for the simultaneous determination of tetracyclines, macrolides, quinolones, sulphonamides and anthelmintics (including benzimidazoles and avermectins) in eggs by ultra-high pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Solvent extraction. solid-phase extraction (SPE). matrix solid-phase dispersion (MSPD) and modified QuEChERS procedure were compared in terms of recovery and number of veterinary drugs extracted. The solvent extraction procedure with a clean-up step provided better results than the other tested procedures. The QuEChERS procedure was simpler and faster, but extracted fewer compounds than solvent extraction. MSPD did not extract tetracyclines and quinolones, whereas macrolides and tetracyclines were not extracted when SPE was applied. The solvent extraction procedure was validated, obtaining recoveries ranging from 60% (sulfaquinoxaline) to 119% (levamisole) with repeatability values (expressed as relative standard deviations, RSDs) lower than 20% at two concentration levels (10 and 100 mu g kg(-1)), except for erythromycin, emamectin and ivermectin that showed RSD values close to 25% at 10 mu g kg(-1). Limits of quantification (LOQs) were always equal or lower than 5 mu g kg(-1). Finally the method was applied to egg samples, and erythromycin, enrofloxacin, difloxacin, thiabendazole, emamectin and fenbendazole were detected in four samples. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:150 / 160
页数:11
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