PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis

被引:86
作者
Ishimaru, S
Ueda, R
Hinohara, Y
Ohtani, M
Hanafusa, H
机构
[1] Osaka Biosci Inst, Oncol Mol Lab, Osaka 5650874, Japan
[2] Natl Inst Genet, Genet Strain Res Ctr, Shizuoka, Japan
[3] Mitsubishi Kagaku Inst Life Sci, Tokyo, Japan
关键词
Crk-Mbc-ELMO complex; JNK activation; PVR; Rac; thorax closure;
D O I
10.1038/sj.emboj.7600417
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
PVR, the Drosophila homolog of the PDGF/VEGF receptor, has been implicated in border cell migration during oogenesis and hemocyte migration during embryogenesis. It was earlier shown that Mbc, a CDM family protein, and its effector, Rac, transduced the guidance signal from PVR during border cell migration. Here we demonstrate that PVR is also required for the morphogenetic process, thorax closure, during metamorphosis. The results of genetic and biochemical experiments indicate that PVR activates the JNK pathway. We present evidence showing Crk (an adaptor molecule), Mbc, ELMO (a homolog of Caenorhabditis elegans CED-12 and mammalian ELMO), and Rac to be mediators of JNK activation by PVR. In addition, we suppose that not only Rac but also Cdc42 is activated and involved in JNK activation downstream of PVR.
引用
收藏
页码:3984 / 3994
页数:11
相关论文
共 49 条
[1]  
Agnès F, 1999, DEVELOPMENT, V126, P5453
[2]   αvβ5 integrin recruits the Crkll-Dock180-Rac1 complex for phagocytosis of apoptotic cells [J].
Albert, ML ;
Kim, JI ;
Birge, RB .
NATURE CELL BIOLOGY, 2000, 2 (12) :899-905
[3]   Rho GTPases and their effector proteins [J].
Bishop, AL ;
Hall, A .
BIOCHEMICAL JOURNAL, 2000, 348 (02) :241-255
[4]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[5]   The PDGF/VEGF receptor controls blood cell survival in Drosophila [J].
Brückner, K ;
Kockel, L ;
Duchek, P ;
Luque, CM ;
Rorth, P ;
Perrimon, N .
DEVELOPMENTAL CELL, 2004, 7 (01) :73-84
[6]   Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex [J].
Brugnera, E ;
Haney, L ;
Grimsley, C ;
Lu, MJ ;
Walk, SF ;
Tosello-Trampont, AC ;
Macara, IG ;
Madhani, H ;
Fink, GR ;
Ravichandran, KS .
NATURE CELL BIOLOGY, 2002, 4 (08) :574-582
[7]   Visualization of gene expression in living adult Drosophila [J].
Calleja, M ;
Moreno, E ;
Pelaz, S ;
Morata, G .
SCIENCE, 1996, 274 (5285) :252-255
[8]   Developmental control of blood cell migration by the Drosophila VEGF pathway [J].
Cho, NK ;
Keyes, L ;
Johnson, E ;
Heller, J ;
Ryner, L ;
Karim, F ;
Krasnow, MA .
CELL, 2002, 108 (06) :865-876
[9]   Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways [J].
Clemens, JC ;
Worby, CA ;
Simonson-Leff, N ;
Muda, M ;
Maehama, T ;
Hemmings, BA ;
Dixon, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6499-6503
[10]   Guidance of cell migration by the Drosophila PDGF/VEGF receptor [J].
Duchek, P ;
Somogyi, K ;
Jékely, G ;
Beccari, S ;
Rorth, P .
CELL, 2001, 107 (01) :17-26