Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells

被引:534
作者
Haines-Butterick, Lisa
Rajagopal, Karthikan
Branco, Monica
Salick, Daphne
Rughani, Ronak
Pilarz, Matthew
Lamm, Matthew S.
Pochan, Darrin J. [1 ]
Schneider, Joel P.
机构
[1] Univ Delaware, Delaware Biotechnol Inst, Dept Chem & Biochem, Newark, DE 19716 USA
[2] Univ Delaware, Delaware Biotechnol Inst, Dept Mat Sci & Engn, Newark, DE 19716 USA
关键词
hydrogel; self-assembly; stem cell;
D O I
10.1073/pnas.0701980104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A peptide-based hydrogelation strategy has been developed that allows homogenous encapsulation and subsequent delivery of C3H10t1/2 mesenchymal stem cells. Structure-based peptide design afforded MAX8, a 20-residue peptide that folds and self-assembles in response to DMEM resulting in mechanically rigid hydrogels. The folding and self-assembly kinetics of MAX8 have been tuned so that when hydrogelation is triggered in the presence of cells, the cells become homogeneously impregnated within the gel. A unique characteristic of these gel-cell constructs is that when an appropriate shear stress is applied, the hydrogel will shear-thin resulting in a low-viscosity gel. However, after the application of shear has stopped, the gel quickly resets and recovers its initial mechanical rigidity in a near quantitative fashion. This property allows gel/cell constructs to be delivered via syringe with precision to target sites. Homogenous cellular distribution and cell viability are unaffected by the shear thinning process and gel/cell constructs stay fixed at the point of introduction, suggesting that these gels may be useful for the delivery of cells to target biological sites in tissue regeneration efforts.
引用
收藏
页码:7791 / 7796
页数:6
相关论文
共 45 条
[21]   Cytocompatibility of self-assembled ß-hairpin peptide hydrogel surfaces [J].
Kretsinger, JK ;
Haines, LA ;
Ozbas, B ;
Pochan, DJ ;
Schneider, JP .
BIOMATERIALS, 2005, 26 (25) :5177-5186
[22]   Laminated morphology of nontwisting β-sheet fibrils constructed via peptide self-assembly [J].
Lamm, MS ;
Rajagopal, K ;
Schneider, JP ;
Pochan, DJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (47) :16692-16700
[23]   Hydrogels for tissue engineering [J].
Lee, KY ;
Mooney, DJ .
CHEMICAL REVIEWS, 2001, 101 (07) :1869-1879
[24]   Exploiting amyloid fibril lamination for nanotube self-assembly [J].
Lu, K ;
Jacob, J ;
Thiyagarajan, P ;
Conticello, VP ;
Lynn, DG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (21) :6391-6393
[25]   Soft-tissue augmentation with injectable alginate and syngeneic fibroblasts [J].
Marler, JJ ;
Guha, A ;
Rowley, J ;
Koka, R ;
Mooney, D ;
Upton, J ;
Vacanti, JP .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2000, 105 (06) :2049-2058
[26]   Photopolymerizable hydrogels for tissue engineering applications [J].
Nguyen, KT ;
West, JL .
BIOMATERIALS, 2002, 23 (22) :4307-4314
[27]  
Oerther S, 1999, BIOTECHNOL BIOENG, V63, P206, DOI 10.1002/(SICI)1097-0290(19990420)63:2<206::AID-BIT9>3.3.CO
[28]  
2-#
[29]   Semiflexible chain networks formed via self-assembly of β-hairpin molecules -: art. no. 268106 [J].
Ozbas, B ;
Rajagopal, K ;
Schneider, JP ;
Pochan, DJ .
PHYSICAL REVIEW LETTERS, 2004, 93 (26)
[30]   Salt-triggered peptide folding and consequent self-assembly into hydrogels with tunable modulus [J].
Ozbas, B ;
Kretsinger, J ;
Rajagopal, K ;
Schneider, JP ;
Pochan, DJ .
MACROMOLECULES, 2004, 37 (19) :7331-7337