Intracellular mapping of 4′-deoxy-4′-iododoxorubicin in sensitive and multidrug resistant cells by electron spectroscopic imaging

被引：3

作者：

Diociaiuti, M ^{[1
]}

Calcabrini, A ^{[1
]}

Meschini, S ^{[1
]}

Arancia, G ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Ultrastruct, I-00161 Rome, Italy

来源：

MICRON | 1997年 / 28卷 / 05期

关键词：

electron energy loss spectroscopy; electron spectroscopic imaging; laser scanning confocal microscopy; iododoxorubicin; multidrug resistance;

D O I：

10.1016/S0968-4328(97)00047-4

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Electron spectroscopic imaging (ESI) was employed to study, with high spatial resolution, the intracellular distribution of the halogenated derivative of doxorubicin 4'-deoxy-4'-iododoxorubicin (IDX) in sensitive and multidrug resistant human breast carcinoma cells. Both ESI and electron energy loss spectroscopy (EELS) observations confirmed results obtained with flow cytometry (FC), laser scanning confocal microscopy (LSCM) and secondary ion mass spectrometry (SIMS). Moreover, ESI allowed us to obtain a more detailed intracellular localization of IDX. Our results confirm that nuclear DNA represents the main intracellular target for IDX and that the Golgi apparatus is involved in the intracellular transport of the drug. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：389 / 395

页数：7

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