Advances in determination of vitamin D related compounds in biological samples using liquid chromatography-mass spectrometry: A review

被引：87

作者：

Higashi, Tatsuya ^{[1
]}

Shimada, Kazutake ^{[2
]}

Toyo'oka, Toshimasa ^{[1
]}

机构：

[1] Univ Shizuoka, Sch Pharmaceut Sci, Suruga Ku, Shizuoka 4228526, Japan

[2] Kanazawa Univ, Aoba Ku, Sendai, Miyagi 9800822, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 20期

关键词：

Vitamin D metabolite; Synthetic vitamin D analogue; Biological sample; Liquid chromatography-mass spectrometry; Diagnostic assessment; Derivatization; DIELS-ALDER DERIVATIZATION; 25-HYDROXYVITAMIN D-3; HUMAN PLASMA; 1-ALPHA; 25-DIHYDROXYVITAMIN D-3; HUMAN SERUM; (24R)-24,25-DIHYDROXYVITAMIN D-3; 24,25-DIHYDROXYVITAMIN D-3; C-3; EPIMERIZATION; D ANALOGS; PERFORMANCE;

D O I：

10.1016/j.jchromb.2009.11.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurements of the serum/plasma concentrations of vitamin D metabolites are widely used for the diagnostic assessment and follow-up of several diseases, such as chronic renal failure and osteoporosis These metabolites have usually been measured by protein binding assays, such as radioimmunoassay and radioreceptor assay Although these techniques will doubtless continue to be the methods of choice for routine use in the clinical field, their specificity and accuracy are sometimes poor due to interference from other metabolites and lipids Among the alternative methods, liquid chromatography (LC) coupled with mass spectrometry (MS) has been used for the analysis of these metabolites and even synthetic vitamin D analogues (therapeutic agents) due to its sensitivity and selectivity This article reviews recent advances in the determination of vitamin D metabolites and related compounds in biological samples using LC-MS (C) 2009 Elsevier B V All rights reserved

引用

页码：1654 / 1661

页数：8

共 67 条

[1] DIFFERENTIATION OF MOUSE MYELOID-LEUKEMIA CELLS INDUCED BY 1-ALPHA,25-DIHYDROXYVITAMIN-D3 [J].