Isolation and physicochemical characterization of soluble scleroglucan from Sclerotium rolfsii.: Rheological properties, molecular weight and conformational characteristics

被引:77
作者
Fariña, JI
Siñeriz, F
Molina, OE
Perotti, NI
机构
[1] PROIMI Planta Piloto Proc Ind Microbiol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Univ Nacl Tucuman, Catedra Microbiol Super, Fac Bioq Qca & Fcia, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[3] Univ Nacl Tucuman, Catedra Microbiol Gral & Ind, Fac Cs Exactas & Tecnol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
关键词
Sclerotium rolfsii; rheological properties; conformation;
D O I
10.1016/S0144-8617(00)00189-2
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Scleroglucan produced by Sclerotium rolfsii ATCC 201126 exhibited pseudoplastic behaviour and an exponential relationship between apparent viscosity and polymer concentration. Viscosity showed strong heat-stability (100 degreesC/60 min). Tolerance up to 20% w/v NaCl and over a wide pH range (0-13) was also found. A sharp decrease on viscosity at NaOH concentrations higher than 0.15 M was observed due to dissociation of the triple-helix. According to intrinsic viscosity determinations and gel filtration chromatography on Sepharose CL-4B, the weight-average molecular weight of native polysaccharide was about 5 x 10(6) Da for the tripler and 1.6 x 10(6) Da for the random coil. These values, in addition to the change in complexation with Congo Red, support the idea of the triple-helical conformation in neutral or slightly alkaline solutions (<0.15 M NaOH), and single chains in a highly alkaline environments. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:41 / 50
页数:10
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