Heptakis(6-amino-6-deoxy)-β-cyclodextrin as a chiral selector for the separation of anionic analyte enantiomers by capillary electrophoresis

被引：23

作者：

Budanova, N

Shapovalova, E

Lopatin, S

Varlamov, V

Shpigun, O

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Div Analyt Chem, Moscow, Russia

[2] Russian Acad Sci, Ctr Bioengn, Moscow, Russia

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 16期

关键词：

capillary electrophoresis; carboxybenzyl-amino acid; carboxylic acid; cationic beta-cyclodextrin; enantiomer separation;

D O I：

10.1002/elps.200405970

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A hepta-substituted beta-cyclodextrin bearing seven amino groups, heptakis(6-amino-6-deoxy)-beta-cyclodextrin (per-6-NH2-beta-CD) was successfully used as a chiral selector for the enantioseparation of different anionic analytes. The running buffer pH and chiral selector concentration were the studied parameters crucial in achieving the maximum possible enantioresolution. Enantiomeric separation of a mixture of seven carboxy-benzyl-amino acids was achieved in 24 min. Excellent resolution was obtained for carboxybenzyl-tryptophan (R-s = 11.2).

引用

页码：2795 / 2800

页数：6

共 46 条

[31] Fast development of separation methods for the chiral analysis of amino acid derivatives using capillary electrophoresis and experimental designs [J].