Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria

被引：62

作者：

De Leonardis, S ^{[1
]}

Dipierro, N ^{[1
]}

Dipierro, S ^{[1
]}

机构：

[1] Univ Bari, Dept Plant Biol & Pathol, Plant Physiol Lab, I-70126 Bari, Italy

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2000年 / 38卷 / 10期

关键词：

ascorbate; ascorbate peroxidase; mitochondria; reactive oxygen species; Solanum tuberosum;

D O I：

10.1016/S0981-9428(00)01188-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ascorbate peroxidase (APX) has been purified from potato tuber (Solanum tuberosum L.) mitochondria. The potato enzyme appeared to be localized inside mitochondria. The mitochondrial APX was purified to homogeneity and its physico-chemical and kinetic propel-ties were compared with those of the cytosolic enzyme. The molecular mass of mAPX was 31 kDa, as estimated by SDS-PAGE, and was similar to that of the cytosolic enzyme, but its relative mobility in non-denaturing PAGE was different from the cytosolic APX. The K-m values of mAPX for AsA and H2O2 were 76.1 +/- 23.1 and 80.3 +/- 24.9 muM, respectively, and were higher than those of the cytosolic enzyme. Mitochondrial APX was sensitive to inhibition by sulfhydryl reagents, such as mersalyl and p-hydroxymercuribenzoate (p-HMB). A role for the mitochondrial ascorbate-ascorbate peroxidase system in the scavenging of toxic oxygen species inside potato tuber mitochondria is proposed. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

引用

页码：773 / 779

页数：7

共 35 条

[1]

AMAKO K, 1994, PLANT CELL PHYSIOL, V35, P497

[2]

Asada K., 1994, Causes of photooxidative stress and amelioration of defense systems in plants., P77

[3] Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV [J].

Bartoli, CG ;

Pastori, GM ;

Foyer, CH .

PLANT PHYSIOLOGY, 2000, 123 (01) :335-343

[4]

BOWLER C, 1994, SUPEROXIDE DISMUTASE

[5] Ascorbate peroxidase - A prominent membrane protein in oilseed glyoxysomes [J].

Bunkelmann, JR ;

Trelease, RN .

PLANT PHYSIOLOGY, 1996, 110 (02) :589-598

[6] SEPARATION OF MITOCHONDRIA FROM MICROBODIES OF PISUM-SATIVUM (L-CV ALASKA) COTYLEDONS [J].

BURGESS, N ;

BEAKES, GW ;

THOMAS, DR .

PLANTA, 1985, 166 (02) :151-155

[7] Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli [J].

Caldwell C.R. ;

Turano F.J. ;

McMahon M.B. .

Planta, 1997, 204 (1) :120-126

[8]

CHEN GX, 1992, PLANT CELL PHYSIOL, V33, P117

[9]

CHEN GX, 1989, PLANT CELL PHYSIOL, V30, P987

[10] GLUTAMATE DEHYDROGENASE FROM PUMPKIN COTYLEDONS - CHARACTERIZATION AND ISOENZYMES [J].

CHOU, KH ;

SPLITTSTOESSER, WE .

PLANT PHYSIOLOGY, 1972, 49 (04) :550-+

← 1 2 3 4 →