Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis

被引:71
作者
Boonla, Chanchai
Wunsuwan, Rattiporn
Tungsanga, Kriang
Tosukhowong, Piyaratana
机构
[1] Chulalongkorn Univ, Fac Med, Dept Biochem, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Fac Med, Biochem & Mol Biol Metab Dis Res Unit, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Fac Med, Dept Med, Bangkok 10330, Thailand
来源
UROLOGICAL RESEARCH | 2007年 / 35卷 / 04期
关键词
nephrolithiasis; kidney stone; oxidative stress; 8-OHdG; ROC analysis; diagnostic values;
D O I
10.1007/s00240-007-0098-0
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
8-hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified guanosine, which has been widely used as an oxidative DNA damage marker in various diseases. The present study aimed to determine urinary 8-OHdG in nephrolithiasis patients and evaluate its clinical significance. Thirty-six nephrolithiasis patients and 30 healthy subjects were recruited. Urine volume, creatinine, malondialdehyde, fl-N-acetylglucosaminidase (NAG) activity and proteins were measured in 24 h urine samples. Urinary 8OHdG was determined by competitive enzyme-linked immunosorbent assay. Mineral composition of stones was analyzed using Fourier-transformed infrared spectroscopy. Nephrolithiasis patients excreted urinary 8-OHdG significantly higher than healthy controls. Urinary 8-OHdG levels compared among patients with calcium oxalate, struvite and uric acid stones were insignificantly different. The urinary NAG activity correlated positively with urinary 8OHdG. Multiple linear regression showed that urinary NAG activity was an independent predictor of urinary 8OHdG level. Receiver operating characteristic analysis revealed that the urinary 8-OHdG test was adequate for diagnosing nephrolithiasis. At 10 mu glg creatinine cutoff, the 8-OHdG test imparted high specificity (96.67%) and a positive predictive value (91.67%). In conclusion, this is the first report of elevated urinary 8-OHdG excretion in nephrolithiasis patients indicating increased oxidative DNA damage. Increased renal tubular damage was independently associated with elevated urinary 8-OHdG. Elevated urinary 8-OHdG levels adjunct with metabolic profile may be useful for identifying people at risk of stone development.
引用
收藏
页码:185 / 191
页数:7
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