Bone regeneration by implantation of adipose-derived stromal cells expressing BMP-2

被引：114

作者：

Li, Huiwu

Dai, Kerong

Tang, Tingting

Zhang, Xiaoling

Yan, Mengning

Lou, Jueren

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Dept Orthopaed Surg, Peoples Hosp 9, Shanghai 200011, Peoples R China

[2] CAS, SIBS, Hlth & Sci Ctr, Shanghai 200025, Peoples R China

[3] SSMU, Shanghai 200025, Peoples R China

[4] Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 04期

基金：

中国国家自然科学基金;

关键词：

mesenchymal stem cells; adipose tissue; bone morphogenetic proteins; gene therapy; bone repair; cell differentiation; dogs; ulna; animal experimentation; biocompatible materials;

D O I：

10.1016/j.bbrc.2007.02.165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we reported that the adipose-derived stromal cells (ADSCs) genetically modified by bone morphogenetic protein 2 (BMP-2) healed critical-sized canine ulnar bone defects. First, the osteogenic and adipogenic differentiation potential of the ADSCs derived from canine adipose tissue were demonstrated. And then the cells were modified by the BMP-2 gene and the expression andbone-induction ability of BMP-2 were identified. Finally, the cells modified by BMP-2 gene were applied to a beta-tricalcium phosphate (TCP) carrier and implanted into ulnar bone defects in the canine model. After 16 weeks, radiographic, histological, and histomorphometry analysis showed that ADSCs modified by BMP-2 gene produced a significant increase of newly formed bone area and healed or partly healed all of the bone defects. We conclude that ADSCs modified by the BMP-2 gene can enhance the repair of critical-sized bone defects in large animals. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：836 / 842

页数：7

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