Reverse transcription-booster PCR for detection of noroviruses in shellfish

被引：26

作者：

De Medici, D ^{[1
]}

Croci, L ^{[1
]}

Suffredini, E ^{[1
]}

Toti, L ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Alimenti, I-00161 Rome, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 10期

关键词：

D O I：

10.1128/AEM.70.10.6329-6332.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RTPCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NY strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.

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页码：6329 / 6332

页数：4

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