Directed cosmid isolation of bovine markers for physical assignment by fish

被引：9

作者：

Heaton, MP

LopezCorrales, NL

Smith, TPL

Kappes, SM

Beattie, CW

机构：

[1] USDA, ARS, Roman L. Hruska U.S. Meat Anim. R., Clay Center

来源：

ANIMAL BIOTECHNOLOGY | 1997年 / 8卷 / 02期

关键词：

D O I：

10.1080/10495399709525879

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A directed PCR-based iterative screening protocol was developed to isolate cosmids containing microsatellite markers linked to chromosomal regions of interest, such as those near the ends of linkage groups or quantitative trait loci. This method was optimized for large-scale screening of total genomic libraries and used to purify bovine cosmids that anchor the ends of the bovine linkage group 28 (BTA28). Cosmids containing ms markers RBP3 and BMS2060 were purified for fluorescence in situ hybridization and assigned to 28q18-q19 and 28q12, respectively. These assignments indicated that approximately 73% of BTA28 (90% excluding the centromere) is covered by the current linkage map. Since this method is applicable to any target gene sequence suitable for PCR amplification, it may be extended to comparative mapping of genes.

引用

页码：167 / 177

页数：11

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