Genes coding for respiratory complexes map on all three chromosomes of the Paracoccus denitrificans genome

被引:22
作者
Winterstein, C [1 ]
Ludwig, B [1 ]
机构
[1] Inst Biochem, Biozentrum, D-60439 Frankfurt, Germany
关键词
pulsed-field gel electrophoresis; megaplasmid; Thiosphaera pantotropha; Paracoccus denitrificans; genome size;
D O I
10.1007/s002030050572
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genome of Paracoccus denitrificans (strain Pd1222) consists of three distinct DNA molecules when separated by standard pulsed-field gel electrophoresis with apparent molecular sizes of approximately 2, 1.1, and 0.64 Mb. When the separated chromosomes are digested by restriction enzymes and sizes of resulting fragments are summed up, the three chromosomes are composed of 1.83, 1.16, and 0.67 Mb. Since their migration behavior relative to size standards is largely independent of electrophoresis conditions, at least the two smaller chromosomes most likely represent linear molecules. The size analysis presented here allows an unequivocal distinction between groups of different strains of P. denitrificans and of Thiosphaera pantotropha, confirming an earlier cytochrome c analysis. When the genome was analyzed with different probes coding for respiratory enzymes, essential genes were found spread over all three chromosomes without any obvious clustering on any of the three forms.
引用
收藏
页码:275 / 281
页数:7
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