Multiplex assays for biomarker research and clinical application: Translational science coming of age

被引：73

作者：

Fu, Qin ^{[2
]}

Schoenhoff', Florian S. ^{[2
,3
]}

Savage, William J. ^{[4
,5
]}

Zhang, Pingbo ^{[2
]}

Van Eyk, Jennifer E. ^{[1
,2
]}

机构：

[1] Johns Hopkins Univ, Johns Hopkins Bayview Prote Ctr, Hopkins ITCR Biomarker Dev Grp, Baltimore, MD 21224 USA

[2] Johns Hopkins Univ, Dept Med, Baltimore, MD 21224 USA

[3] Univ Bern, Dept Cardiovasc Surg, Bern, Switzerland

[4] Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21224 USA

[5] Johns Hopkins Univ, Dept Pediat, Baltimore, MD 21224 USA

来源：

PROTEOMICS CLINICAL APPLICATIONS | 2010年 / 4卷 / 03期

基金：

瑞士国家科学基金会; 美国国家卫生研究院;

关键词：

Biomarker; Immunoassay; Multiple reaction monitoring; Multiplex assay; Quantitation; TARGETED MASS-SPECTROMETRY; ANTIBODY ARRAYS; MICROARRAY TECHNOLOGY; BIOANALYTICAL METHODS; PROTEIN MICROARRAYS; TROPONIN-I; PROTEOMICS; VALIDATION; IMMUNOASSAYS; PLASMA;

D O I：

10.1002/prca.200900217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the last decade, translational science has come into the focus of academic medicine, and significant intellectual and financial efforts have been made to initiate a multitude of bench-to-bedside projects. The quest for suitable biomarkers that will significantly change clinical practice has become one of the biggest challenges in translational medicine. Quantitative measurement of proteins is a critical step in biomarker discovery. Assessing a large number of potential protein biomarkers in a statistically significant number of samples and controls still constitutes a major technical hurdle. Multiplexed analysis offers significant advantages regarding time, reagent cost, sample requirements and the amount of data that can be generated. The two contemporary approaches in multiplexed and quantitative biomarker validation, antibody-based immunoassays and MS-based multiple (or selected) reaction monitoring, are based on different assay principles and instrument requirements. Both approaches have their own advantages and disadvantages and therefore have complementary roles in the multi-staged biomarker verification and validation process. In this review, we discuss quantitative immunoassay and multiple reaction monitoring/selected reaction monitoring assay principles and development. We also discuss choosing an appropriate platform, judging the performance of assays, obtaining reliable, quantitative results for translational research and clinical applications in the biomarker field.

引用

页码：271 / 284

页数：14

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