Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis

被引：26

作者：

Tang, XM

Lakay, FM

Shen, W

Shao, WL

Fang, HY

Prior, BA

Wang, ZX

Zhuge, J

机构：

[1] Univ Stellenbosch, Dept Microbiol, ZA-7602 Matieland, South Africa

[2] So Yangtze Univ, Key Lab Ind Biotechnol, Minist Educ, Res Ctr Ind Microbiol, Wuxi 214036, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 18期

关键词：

alkaline protease; Bacillus licheniformis; characterisation; purification;

D O I：

10.1023/B:BILE.0000045642.19299.3f

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60degreesC (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition.

引用

页码：1421 / 1424

页数：4

共 8 条

[1] CATHEPSIN DEGRADATION OF PACIFIC WHITING SURIMI PROTEINS [J].