Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species

被引：79

作者：

Koh, MC ^{[1
]}

Lim, CH ^{[1
]}

Chua, SB ^{[1
]}

Chew, ST ^{[1
]}

Phang, STW ^{[1
]}

机构：

[1] Primary Prod Dept, Vet Publ Hlth Lab, Singapore 619495, Singapore

来源：

MEAT SCIENCE | 1998年 / 48卷 / 3-4期

关键词：

D O I：

10.1016/S0309-1740(97)00104-6

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The random amplified polymorphic DNA (RAPD) method was used to generate fingerprint patterns for 10 meat species: wild boar, pig, horse, buffalo, beef, venison, dog, cat, rabbit and kangaroo. A total of 29 10-nucleotide primers, with GC contents ranging from 50-80%, were evaluated for their specificity and efficiency. The fingerprint patterns that were generated were found in some cases to be species-specific, i.e. one species could be differentiated fr om another. The advantages and disadvantages of using RAPD-PCR for the identification of red meat species are also discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：275 / 285

页数：11

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