NAAG reduces NMDA receptor current in CA1 hippocampal pyramidal neurons of acute slices and dissociated neurons

被引:53
作者
Bergeron, R
Coyle, JT
Tsai, GC
Greene, RW
机构
[1] Univ Ottawa, Ottawa Hlth Res Inst, Dept Psychiat, Ottawa, ON K1Y 4E9, Canada
[2] Harvard Univ, Sch Med, Dept Psychiat, Neurosci Lab, Belmont, MA 02178 USA
关键词
synaptic transmission; NMDA receptor; glycine; 7-chlorokynurenic acid; patch-clamp; hippocampus;
D O I
10.1038/sj.npp.1300559
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
N-Acetylaspartylglutamate (NAAG) is an abundant neuropeptide in the nervous system, yet its functions are not well understood. Pyramidal neurons of the CA1 sector of acutely prepared hippocampal slices were recorded using the whole-cell patch-clamp technique. At low concentrations (20 muM), NAAG reduced isolated N-methyl-D-aspartate receptor (NMDAR)-mediated synaptic currents or NMDA-induced currents. The NAAG-induced change in the NMDA concentration/response curve suggested that the antagonism was not competitive. However, the NAAG-induced change in the concentration/response curve for the NMDAR co-agonist, glycine, indicated that glycine can overcome the NAAG antagonism. The antagonism of the NMDAR induced by NAAG was still observed in the presence of LY-341495, a potent and selective mGluR3 antagonist. Moreover, in dissociated pyramidal neurons of the CA1 region, NAAG also reduced the NMDA current and this effect was reversed by glycine. These results suggest that NAAG reduces the NMDA currents in hippocampal CA1 pyramidal neurons.
引用
收藏
页码:7 / 16
页数:10
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