Rat α-synuclein interacts with Tat binding protein 1, a component of the 26S proteasomal complex

被引：88

作者：

Ghee, M

Fournier, A

Mallet, J

机构：

[1] Hop La Pitie Salpetriere, Lab Gent Mol Neurotransmiss & Proc Neurdegenerat, CNRS, UMR 9923, F-75013 Paris, France

[2] Aventis Pharma Res & Dev, Vitry Sur Seine, France

来源：

JOURNAL OF NEUROCHEMISTRY | 2000年 / 75卷 / 05期

关键词：

alpha-synuclein; Parkinson's disease; Tat binding protein 1;

D O I：

10.1046/j.1471-4159.2000.0752221.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The alpha-synuclein gene, which encodes a brain presynaptic nerve terminal protein of unknown function, is linked to familial early-onset Parkinson's disease (PD). The finding that alpha-synuclein forms the major fibrillary component of Lewy bodies in brains of PD patients suggests that the two point mutations in alpha-synuclein (Ala(53)Thr, Ala(30)Pro) may promote the aggregation of alpha-synuclein into filaments. To address the role of alpha-synuclein in neurodegenerative diseases, we performed a yeast two-hybrid screen of a rat adult brain cDNA library using rat alpha-synuclein 2 (alpha SYN2). Here we report that alpha SYN2 interacts specifically with Tat binding protein 1, a subunit of the 700-kDa proteasome activator (PA700), the regulatory complex of the 26S proteasome and of the modulator complex, which enhances PA700 activation of the proteasome.

引用

页码：2221 / 2224

页数：4

共 30 条

[1] Structural and functional effects of PA700 and modulator protein on proteasomes [J].

Adams, GM ;

Falke, S ;

Goldberg, AL ;

Slaughter, CA ;

DeMartino, GN ;

Gogol, EP .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (03) :646-657

[2]

Bartel P, 1993, CELLULAR INTERACTION, P153

[3] Degradation of α-synuclein by proteasome [J].

Bennett, MC ;

Bishop, JF ;

Leng, Y ;

Chock, PB ;

Chase, TN ;

Mouradian, MM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (48) :33855-33858

[4] Intrastriatal injection of an adenoviral vector expressing glial-cell-line-derived neurotrophic factor prevents dopaminergic neuron degeneration and behavioral impairment in a rat model of Parkinson disease [J].

BilangBleuel, A ;

Revah, F ;

Colin, P ;

Locquet, I ;

Robert, JJ ;

Mallet, J ;

Horellou, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (16) :8818-8823

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] Accelerated in vitro fibril formation by a mutant α-synuclein linked to early-onset Parkinson disease [J].

Conway, KA ;

Harper, JD ;

Lansbury, PT .

NATURE MEDICINE, 1998, 4 (11) :1318-1320

[7]

DEMARTINO GN, 1994, J BIOL CHEM, V269, P20878

[8] Identification, purification, and characterization of a PA700-dependent activator of the proteasome [J].

DeMartino, GN ;

Proske, RJ ;

Moomaw, CR ;

Strong, AA ;

Song, XL ;

Hisamatsu, H ;

Tanaka, K ;

Slaughter, CA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (06) :3112-3118

[9]

DEVERAUX Q, 1994, J BIOL CHEM, V269, P7059

[10] TAT-BINDING PROTEIN-7 IS A SUBUNIT OF THE 26S PROTEASE [J].

DUBIEL, W ;

FERRELL, K ;

RECHSTEINER, M .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1994, 375 (04) :237-240

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