The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes

被引：37

作者：

Kim, Dong-Hwan

Smith, Jason T.

Chilkoti, Ashutosh

Reichert, William M.

机构：

[1] Department of Biomedical Engineering, Duke University, Durham, NC 27708

来源：

BIOMATERIALS | 2007年 / 28卷 / 23期

关键词：

IL-1ra; monocytes; cytokines; inflammation; wound healing; INTERLEUKIN-1 RECEPTOR ANTAGONIST; TUMOR-NECROSIS-FACTOR; SURFACE-PLASMON RESONANCE; MONONUCLEAR-CELLS INVITRO; GROWTH-FACTOR; MESSENGER-RNA; LIGAND-BINDING; BIOMATERIALS; INDUCTION; RESPONSES;

D O I：

10.1016/j.biomaterials.2007.04.010

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The objective of this research was to investigate whether immobilized anti-inflammatory cytokines will signal changes in the inflammatory profile of cultured monocytes. A fusion protein of recombinant human IL-I receptor antagonist and elastin-like peptide (IL-1ra-ELP) was expressed in Escherichia coh. THP-1 human monocytes were cultured on either carboxyl-terminated self-assembled monolayers (SAMs), or SAMs with either covalently immobilized or soluble IL-1ra-ELP. LPS-stimulated monocytes exposed to either soluble or immobilized IL-1ra-ELP were prevented from cell differentiation, showed attenuated expression of pro-inflammatory cytokines, and had increased production of anti-inflammatory and pro-wound healing cytokines. These results suggest that immobilized anti-inflammatory cytokines have the potential to be immunomodulatory biomaterials. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3369 / 3377

页数：9

共 48 条

[11] Ultra-high expression of a thermally responsive recombinant fusion protein in E-coli [J].