C3d enhancement of antibodies to hemagglutinin accelerates protection against influenza virus challenge

被引：192

作者：

Ross, TM ^{[1
]}

Xu, Y ^{[1
]}

Bright, RA ^{[1
]}

Robinson, HL ^{[1
]}

机构：

[1] Emory Univ, Div Microbiol & Immunol, Yerkes Reg Primate Res Ctr, Atlanta, GA 30329 USA

来源：

NATURE IMMUNOLOGY | 2000年 / 1卷 / 02期

关键词：

D O I：

10.1038/77802

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The ability of the C3d component of complement to enhance antibody responses and protective immunity to influenza virus challenges was evaluated using a DNA vaccine encoding a C3d fusion of the hemagglutinin (HA) from influenza virus, Plasmids were generated that encoded a transmembrane HA (tmHA), a secreted form of HA (sHA), or a sHA fused to three tandem copies of the murine homologue of the C3d (sHA-3C3d). Analysis of the titers, avidity maturation, and hemagglutinin-inhibition activity of raised antibody revealed that immunizations with sHA-3C3d DNA accelerated both the avidity maturation of antibody to HA and the appearance of hemagglutinin-inhibition activity. These accelerated antibody responses correlated to a more rapid appearance of protective immunity. They also correlated to complete protection from live virus challenge by a single vaccination at a dose ten times lower than the protective dose for non-C3d forms of HA.

引用

页码：127 / 131

页数：5

共 26 条

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