Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing

被引：82

作者：

Brunner, J ^{[1
]}

Kraemer, R ^{[1
]}

机构：

[1] Heidelberg Univ, Anorgan Chem Inst, D-69120 Heidelberg, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 42期

关键词：

D O I：

10.1021/ja047252a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3′-end and a copper(II) complex to the 5′-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches. Copyright © 2004 American Chemical Society.

引用

页码：13626 / 13627

页数：2

共 26 条

[1] Structure of a copper-mediated base pair in DNA [J].

Atwell, S ;

Meggers, E ;

Spraggon, G ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (49) :12364-12367

[2] DNA-templated metal catalysis [J].

Brunner, J ;

Mokhir, A ;

Kraemer, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (41) :12410-12411

[3] Fluorescent sensors for transition metals based on electron-transfer and energy-transfer mechanisms [J].

Fabbrizzi, L ;

Licchelli, M ;

Pallavicini, P ;

Perotti, A ;

Taglietti, A ;

Sacchi, D .

CHEMISTRY-A EUROPEAN JOURNAL, 1996, 2 (01) :75-82

[4]

Gutierrez A. F., 1985, MOL LUMINESCENCE SPE, P371

[5] Designing the selectivity of the fluorescent detection of amino acids:: A chemosensing ensemble for histidine [J].

Hortalá, MA ;

Fabbrizzi, L ;

Marcotte, N ;

Stomeo, F ;

Taglietti, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (01) :20-21

[6] Intramolecular dimers: A new design strategy for fluorescence-quenched probes [J].

Johansson, MK ;

Cook, RM .

CHEMISTRY-A EUROPEAN JOURNAL, 2003, 9 (15) :3466-3471

[7] Intramolecular dimers: A new strategy to fluorescence quenching in dual-labeled oligonucleotide probes [J].

Johansson, MK ;

Fidder, H ;

Dick, D ;

Cook, RM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (24) :6950-6956

[8]

KAHN B, 1988, J CHEM SOC DA, P67

[9]

Kramer R., 1998, ANGEW CHEM, V110, P804

[10] Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes [J].

Marras, SAE ;

Kramer, FR ;

Tyagi, S .

NUCLEIC ACIDS RESEARCH, 2002, 30 (21) :e122

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