Genomic analyses of transcription factor binding, histone acetylation, and gene expression reveal mechanistically distinct classes of estrogen-regulated promoters

被引:141
作者
Kininis, Miltiadis
Chen, Benjamin S.
Diehl, Adam G.
Isaacs, Gary D.
Zhang, Tong
Siepel, Adam C.
Clark, Andrew G.
Kraus, W. Lee
机构
[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
[2] Cornell Univ, Field Biochem Mol & Cell Biol, Ithaca, NY 14853 USA
[3] Cornell Univ, Dept Biol Stat & Comp Biol, Ithaca, NY 14853 USA
[4] Cornell Univ, Weill Med Coll, Dept Pharmacol, New York, NY 10021 USA
关键词
D O I
10.1128/MCB.00083-07
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To explore the global mechanisms of estrogen-regulated transcription, we used chromatin immunoprecipitation coupled with DNA microarrays to determine the localization of RNA polymerase II (Poll II), estrogen receptor alpha (ER alpha), steroid receptor coactivator proteins (SRC), and acetyllated histones H-3/H-4 (AcH) at estrogen-regulated promoters in MCF-7 cells with or without estradiol (E2) treatment. In addition, we correlated factor occupancy with gene expression and the presence of transcription factor binding elements. Using this integrative approach, we defined a set of 58 direct E2 target genes based on E2-regulated Pol II occupancy and classified their promoters based on factor binding, histone modification, and transcriptional output. Many of these direct E2 target genes exhibit interesting modes of regulation and biological activities, some of which may be relevant to the onset and proliferation of breast cancers. Our studies indicate that about one-third of these direct E2 target genes contain promoter-proximal ER alpha-binding sites, which is considerably more than previous estimates. Some of these genes represent possible novel targets for regulation through the ER alpha/AP-1 tethering pathway. Our studies have also revealed several previously uncharacterized global features of E2-regulated gene expression, including strong positive correlations between Poll 11 occupancy and AcH levels, as well as between the E2-dependent recruitment of ER alpha and SRC at the promoters of E2-stimulated genes. Furthermore, our studies have revealed new mechanistic insights into E2-regulated gene expression, including the absence of SRC binding at E2-repressed genes and the presence of constitutively bound, promoter-proximally paused Poll IIs at some E2-regulated promoters. These mechanistic insights are likely to be relevant for understanding gene regulation by a wide variety of nuclear receptors.
引用
收藏
页码:5090 / 5104
页数:15
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