Expression of the parathyroid Ca2+-sensing receptor in cytotrophoblasts from human term placenta

被引：40

作者：

Bradbury, RA

Sunn, KL

Crossley, M

Bai, M

Brown, EM

Delbridge, L

Conigrave, AD ^{[1
]}

机构：

[1] Univ Sydney, Dept Biochem G08, Sydney, NSW 2006, Australia

[2] Univ Sydney, Dept Surg, Sydney, NSW 2006, Australia

[3] Brigham & Womens Hosp, Dept Med, Div Endocrine Hypertens, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

JOURNAL OF ENDOCRINOLOGY | 1998年 / 156卷 / 03期

关键词：

D O I：

10.1677/joe.0.1560425

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Fura-2-loaded human cytotrophoblasts responded to elevated extracellular Ca2+ concentration ([Ca2+](o)) with monophasic or, in the case of large (>20 mu m) extravillous cells, biphasic elevations in intracellular free Ca2+ ion concentration ([Ca2+](i)) that returned to baseline levels after restoration of control [Ca2+](o). Large extravillous cytotrophoblasts also responded to elevated [Mg2+](o) with transient elevations in [Ca2+](i), consistent with the behaviour of the parathyroid Ca2+-sensing receptor. Expression of the parathyroid Ca2+-sensing receptor in placental cells was confirmed using Northern blot and reverse transcription (RT)-PCR analysis. However, the major transcript in human placental cells (6.2 kb) differed from that expressed by human parathyroid cells (5.6 kb). RT-PCR analysis and DNA sequencing of key PCR products also revealed the presence of a splice variant in placental and parathyroid cells that lacks exon 3.

引用

页码：425 / 430

页数：6