Cation-chloride cotransporters and GABA-ergic innervation in the human epileptic hippocampus

被引:109
作者
Munoz, Alberto
Mendez, Pablo
DeFelipe, Javier
Alvarez-Leefmans, Francisco Javier
机构
[1] CSIC, Inst Cajal, E-28002 Madrid, Spain
[2] Univ Complutense, Dept Cell Biol, E-28040 Madrid, Spain
[3] Wright State Univ, Dept Pharmacol & Toxicol, Dayton, OH 45435 USA
关键词
epilepsy; subiculum; hippocampal sclerosis; inhibition; NKCC1; KCC2;
D O I
10.1111/j.1528-1167.2007.00986.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Intracellular chloride concentration, [Cl-](i), determines the polarity of GABA(A)-induced neuronal Cl- currents. In neurons, [Cl-](i) is set by the activity of Na+, K+, 2Cl(-) cotransporters (NKCC) such as NKCC1, which physiologically accumulate Cl- in the cell, and Cl- extruding K+, Cl- cotransporters like KCC2. Alterations in the balance of NKCC1 and KCC2 activity may determine the switch from hyperpolarizing to depolarizing effects of GABA, reported in the subiculum of epileptic patients with hippocampal sclerosis. We studied the expression of NKCC (putative NKCC1) and KCC2 in human normal temporal neocortex by Western blot analysis and in normal and epileptic regions of the subiculum and the hippocampus proper using immunocytochemistry. Western blot analysis revealed NKCC and KCC2 proteins in adult human neocortical membranes similar to those in rat neocortex. NKCC and KCC2 immunolabeling of pyramidal and nonpyramidal cells was found in normal and epileptic hippocampal formation. In the transition between the subiculum with sclerotic regions of CA1, known to exhibit epileptogenic activity, double immunolabeling of NKCC and KCC2 revealed that approximately 20% of the NKCC-immunoreactive neurons do not express KCC2. In these same areas some neurons were distinctly hyperinnervated by parvalbumin (PV) positive hypertrophic basket formations that innervated mostly neurons expressing NKCC (74%) and to a lesser extent NKCC-immunonegative neurons (26%). Hypertrophic basket formations also innervated KCC2-positive (76%) and -negative (24%) neurons. The data suggest that changes in the relative expression of NKCC1 and KCC2 in neurons having aberrant GABA-ergic hyperinnervation may contribute to epileptiform activity in the subicular regions adjacent to sclerotic areas of the hippocampus.
引用
收藏
页码:663 / 673
页数:11
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