Diversity of HIV-1 Vpr interactions involves usage of the WXXF motif of host cell proteins

被引:51
作者
BouHamdan, M
Xue, YN
Baudat, Y
Hu, BC
Sire, J
Pomerantz, RJ
Duan, LX [1 ]
机构
[1] Thomas Jefferson Univ, Jefferson Med Coll, Dept Med,Div Infect Dis, Ctr Human Virol,Dorrance H Hamilton Labs, Philadelphia, PA 19107 USA
[2] INSERM, U372, F-13276 Marseille 9, France
关键词
D O I
10.1074/jbc.273.14.8009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Targeting protein or RNA moieties to specific cellular compartments may enhance their desired functions and specificities, Human immunodeficiency virus type I (HIV-1) encodes proteins in addition to Gag, Pol, and Env that are packaged into virus particles, One such retroviral-incorporated protein is Vpr, which is present in all primate lentiviruses, Vpr has been implicated in different roles within the HIV-1 life cycle, In testing a new hypothesis in which viral proteins are utilized as docking sites to incorporate protein moieties into virions, we used the peptide phage display approach to search for Vpr-specific binding peptides, In the present studies, we demonstrate that most of the peptides that bind to Vpr have a common motif, WXXF, More importantly, we demonstrate that the WXXF motif of uracil DNA glycosylase is implicated in the interaction of uracil DNA glycosylase with Vpr intracellularly. Finally, a dimer of the WXXF motif was fused to the chloramphenicol acetyl transferase (CAT) gene, and it was demonstrated that the WXXF dimer-CAT fusion protein construct produces CAT activity within virions in the presence of Vpr as a docking protein, This study provides a novel potential strategy in the targeting of antiviral agents to interfere with HIV-1 replication.
引用
收藏
页码:8009 / 8016
页数:8
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