Kinetic analysis of four HIV-1 reverse transcriptase enzymes mutated in the primer grip region of p66 - Implications for DNA synthesis and dimerization

The highly conserved primer grip region in the p66 subunit of HIV-1 reverse transcriptase (RT) is formed by the beta 12-beta 13 hairpin (residues 227-235), It has been proposed to play a role in aligning the 3'-OH end of the primer in a position for nucleophilic attack on an incoming dNTP, To analyze the importance of the primer grip for RT function, mutant RTs were used that contain single alanine substitutions of residues Trp(229), Met(230), Gly(231), and Tyr(232) in the p66 subunit of the heterodimeric p66/51 enzyme, Steady-state and pre-steady-state kinetic analyses of the enzymes were performed, All mutant enzymes revealed reduced polymerase activity, Mutation of Y232A showed the smallest effect on polymerase function, Equilibrium fluorescence titrations demonstrated that the affinity of the mutants for tRNA was only slightly affected. However, the affinity for primer-template DNA was reduced 27-fold for mutant p66(W229A)/51 and 23-fold for mutant p66(G231A)/51, and the maximal pre-steady-state rate of nucleotide incorporation, k(pol), was reduced 27-fold for p66(W229A)/51 and 70-fold for p66(G231A)/51, respectively, Mutant p66(M230A)/51 revealed no reduced affinity for primer-template but showed a 71-fold reduced affinity for dTTP. Additionally, the mutations Trp(229) and Gly(231) affected the stability of the RT heterodimer.