A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis

被引：54

作者：

Cheng, Yuxiang ^{[1
]}

Long, Mei ^{[1
]}

机构：

[1] NE Forestry Univ, ASNESC, Stress Mol Biol Lab, Harbin 150040, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 07期

关键词：

Arabidopsis thaliana; NADP-malic enzyme; rice (Oryza sativa L.); salt stress;

D O I：

10.1007/s10529-007-9347-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and UV-B radiation. Here, expression of the gene encoding cytosolic NADP-ME (cytoNADP-ME, GenBank Accession No. AY444338) in rice (Oryza sativa L.) seedlings was induced by salt stress (NaCl). NADP-ME activities in leaves and roots of rice also increased in response to NaCl. Transgenic Arabidopsis plants over-expressing rice cytoNADP-ME had a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl. Cytosolic NADPH/NADP(+) concentration ratio of transgenic plants was higher than those of wild-type plants. These results suggest that rice cytoNADP-ME confers salt tolerance in transgenic Arabidopsis seedlings.

引用

页码：1129 / 1134

页数：6

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