Measurement of the NO metabolites, nitrite and nitrate, in human biological fluids by GC-MS

被引：83

作者：

Helmke, Steve M.

Duncan, Mark W.

机构：

[1] Univ Colorado, Hlth Sci Ctr, Dept Pediat, Aurora, CO 80045 USA

[2] Univ Colorado, Hlth Sci Ctr, Div Endocrinol Diabet & Metab, Aurora, CO 80045 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 851卷 / 1-2期

关键词：

reviews; nitration; alkylation; stable isotopes; clinical investigations; mass spectrometry;

D O I：

10.1016/j.jchromb.2006.09.047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In this article we critically review the development and application of gas chromatography-mass spectrometry (GC-MS) techniques to the measurement of the nitric oxide (NO) metabolites, nitrite and nitrate, in human biological fluids. Our focus is on the issue of the fitness of any analytical strategy to its intended purpose and the validity of the analytical results generated. The accuracy, precision, recovery, selectivity and sensitivity of the various methods are evaluated and the potential pitfalls, both specific to the methods, and general to the area, are considered. Several examples of the applications of these techniques to clinical investigations of NO physiology are also critically evaluated. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：83 / 92

页数：10

共 49 条

[1]

Determination of nitric oxide metabolites by means of the Griess assay and gas chromatography-mass spectrometry in the cavernous and systemic blood of healthy males and patients with erectile dysfunction during different functional conditions of the penis [J].