Divergent Requirements for EZH1 in Heart Development Versus Regeneration

被引：72

作者：

Ai, Shanshan ^{[1
]}

Yu, Xianhong ^{[2
]}

Li, Yumei ^{[1
]}

Peng, Yong ^{[2
]}

Li, Chen ^{[1
]}

Yue, Yanzhu ^{[1
]}

Tao, Ge ^{[3
]}

Li, Chuanyun ^{[1
]}

Pu, William T. ^{[4
,5
]}

He, Aibin ^{[1
,2
]}

机构：

[1] Peking Univ, Beijing Key Lab Cardiometabol Mol Med, Inst Mol Med, Beijing 100871, Peoples R China

[2] Peking Univ, Inst Mol Med, Peking Tsinghua Ctr Life Sci, Beijing Key Lab Cardiometabol Mol Med, Beijing, Peoples R China

[3] Baylor Coll Med, Dept Mol Physiol & Biophys, One Baylor Plaza, Houston, TX 77030 USA

[4] Boston Childrens Hosp, Dept Cardiol, Boston, MA USA

[5] Harvard Univ, Harvard Stem Cell Inst, Cambridge, MA 02138 USA

来源：

CIRCULATION RESEARCH | 2017年 / 121卷 / 02期

基金：

中国国家自然科学基金;

关键词：

lysine; methylation; myocardium; regeneration; polycomb repressive complex 2; IMPROVES CARDIAC-FUNCTION; MOUSE HEART; RNA-SEQ; DIFFERENTIATION; METHYLATION;

D O I：

10.1161/CIRCRESAHA.117.311212

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Rationale: Polycomb repressive complex 2 is a major epigenetic repressor that deposits methylation on histone H3 on lysine 27 (H3K27me) and controls differentiation and function of many cells, including cardiac myocytes. EZH1 and EZH2 are 2 alternative catalytic subunits with partial functional redundancy. The relative roles of EZH1 and EZH2 in heart development and regeneration are unknown. Objective: We compared the roles of EZH1 versus EZH2 in heart development and neonatal heart regeneration. Methods and Results: Heart development was normal in Ezh1(-/-) (Ezh1 knockout) and Ezh2(f/f)::cTNT(-Cre) (Ezh2 knockout) embryos. Ablation of both genes in Ezh1(-/-)::Ezh2(f/f)::cTNT(-Cre) embryos caused lethal heart malformations, including hypertrabeculation, compact myocardial hypoplasia, and ventricular septal defect. Epigenome and transcriptome profiling showed that derepressed genes were upregulated in a manner consistent with total EZH dose. In neonatal heart regeneration, Ezh1 was required, but Ezh2 was dispensable. This finding was further supported by rescue experiments: cardiac myocyte-restricted re-expression of EZH1 but not EZH2 restored neonatal heart regeneration in Ezh1 knockout. In myocardial infarction performed outside of the neonatal regenerative window, EZH1 but not EZH2 likewise improved heart function and stimulated cardiac myocyte proliferation. Mechanistically, EZH1 occupied and activated genes related to cardiac growth. Conclusions: Our work unravels divergent mechanisms of EZH1 in heart development and regeneration, which will empower efforts to overcome epigenetic barriers to heart regeneration.

引用

页码：106 / +

页数：22

共 28 条

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