Epithelial cell surface sites involved in the polyvalent adherence of Porphyromonas gingivalis:: a convincing role for neuraminic acid and glucuronic acid

被引：16

作者：

Agnani, G ^{[1
]}

Tricot-Doleux, S ^{[1
]}

Houalet, S ^{[1
]}

Bonnaure-Mallet, M ^{[1
]}

机构：

[1] UPRES EA 1256, Equipe Biol Buccale, F-35000 Rennes, France

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 02期

关键词：

D O I：

10.1128/IAI.71.2.991-996.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the target structures of the epithelial cells responsible for the attachment of Porphyromonas gingivalis by immunocytofluorimetry, enzyme-linked immunosorbent assay, and confocal microscopy. Integrins (beta1, beta3, and alphaV) and E-cadherin played no significant role. Carbohydrates (such as alpha-D-methylglucoside, L-fucose, D- and L-mannose, N-acetylglucosamine, and N-acetylgalactosamine) had little inhibitory effect on bacterial binding. Enzymatic treatments of the epithelial membranes and sugar competition studies showed that N-acetylneuraminic acid and glucuronic acid were involved in binding.

引用

页码：991 / 996

页数：6

共 30 条

[1] Adherence of Porphyromonas gingivalis to gingival epithelial cells:: modulation of bacterial protein expression [J].

Agnani, G ;

Tricot-Doleux, S ;

Du, L ;

Bonnaure-Mallet, N .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 2000, 15 (01) :48-52

[2]

BRUNETTI CR, 1994, J BIOL CHEM, V269, P17067

[3] Integrins αvβ3 and α5β1 mediate attachment of Lyme disease spirochetes to human cells [J].

Coburn, J ;

Magoun, L ;

Bodary, SC ;

Leong, JM .

INFECTION AND IMMUNITY, 1998, 66 (05) :1946-1952

[4] Fimbriae and the hemagglutinating adhesin HA-Ag2 mediate adhesion of Porphyromonas gingivalis to epithelial cells [J].

Du, L ;

PellenMussi, P ;

Chandad, F ;

Mouton, C ;

BonnaureMallet, M .

INFECTION AND IMMUNITY, 1997, 65 (09) :3875-3881

[5] INTERACTION OF MANNOSE-CONTAINING OLIGOSACCHARIDES WITH THE FIMBRIAL LECTIN OF ESCHERICHIA-COLI [J].

FIRON, N ;

OFEK, I ;

SHARON, N .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 105 (04) :1426-1432

[6] Hyaluronan, a possible ligand mediating Treponema denticola binding to periodontal tissue [J].

Haapasalo, M ;

Hannam, P ;

McBride, BC ;

Uitto, VJ .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1996, 11 (03) :156-160

[7] Assessment of internalization and viability of Porphyromonas gingivalis in KB epithelial cells by confocal microscopy [J].

Houalet-Jeanne, S ;

Pellen-Mussi, P ;

Tricot-Doleux, S ;

Apiou, J ;

Bonnaure-Mallet, M .

INFECTION AND IMMUNITY, 2001, 69 (11) :7146-7151

[8] Adherence of Porphyromonas gingivalis to epithelial cells:: analysis by flow cytometry [J].

Huard-Delcourt, A ;

Du, L ;

Pellen-Mussi, P ;

Tricot-Doleux, S ;

Bonnaure-Mallet, M .

EUROPEAN JOURNAL OF ORAL SCIENCES, 1998, 106 (05) :938-944

[9] MULTIPLE BETA-1-CHAIN INTEGRINS ARE RECEPTORS FOR INVASIN, A PROTEIN THAT PROMOTES BACTERIAL PENETRATION INTO MAMMALIAN-CELLS [J].

ISBERG, RR ;

LEONG, JM .

CELL, 1990, 60 (05) :861-871

[10] SPECIFIC-INHIBITION OF ADHERENCE OF AN ORAL-STRAIN OF BACTEROIDES-GINGIVALIS 381 TO EPITHELIAL-CELLS BY MONOCLONAL-ANTIBODIES AGAINST THE BACTERIAL FIMBRIAE [J].

ISOGAI, H ;

ISOGAI, E ;

YOSHIMURA, F ;

SUZUKI, T ;

KAGOTA, W ;

TAKANO, K .

ARCHIVES OF ORAL BIOLOGY, 1988, 33 (07) :479-485

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