Epithelial cell surface sites involved in the polyvalent adherence of Porphyromonas gingivalis:: a convincing role for neuraminic acid and glucuronic acid

被引：16

作者：

Agnani, G ^{[1
]}

Tricot-Doleux, S ^{[1
]}

Houalet, S ^{[1
]}

Bonnaure-Mallet, M ^{[1
]}

机构：

[1] UPRES EA 1256, Equipe Biol Buccale, F-35000 Rennes, France

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 02期

关键词：

D O I：

10.1128/IAI.71.2.991-996.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the target structures of the epithelial cells responsible for the attachment of Porphyromonas gingivalis by immunocytofluorimetry, enzyme-linked immunosorbent assay, and confocal microscopy. Integrins (beta1, beta3, and alphaV) and E-cadherin played no significant role. Carbohydrates (such as alpha-D-methylglucoside, L-fucose, D- and L-mannose, N-acetylglucosamine, and N-acetylgalactosamine) had little inhibitory effect on bacterial binding. Enzymatic treatments of the epithelial membranes and sugar competition studies showed that N-acetylneuraminic acid and glucuronic acid were involved in binding.

引用

页码：991 / 996

页数：6

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