Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA

被引：34

作者：

Ferapontova, Elena E. ^{[1
,2
]}

Hansen, Majken N. ^{[1
,2
]}

Saunders, Aaron M. ^{[3
]}

Shipovskov, Stepan ^{[2
]}

Sutherland, Duncan S. ^{[2
]}

Gothelf, Kurt V. ^{[1
,2
]}

机构：

[1] Aarhus Univ, Dept Chem, Ctr DNA Nanotechnol, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, INANO, DK-8000 Aarhus C, Denmark

[3] Danish Technol Inst, DK-8000 Aarhus C, Denmark

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

HYBRIDIZATION; MICROELECTRODE; BIOSENSORS; METAL;

D O I：

10.1039/b924627d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.

引用

页码：1836 / 1838

页数：3

共 26 条

[1] Double-codified gold nanolabels for enhanced immunoanalysis [J].

Ambrosi, Adriano ;

Castaneda, Maria Teresa ;

Killard, Anthony J. ;

Smyth, Malcolm R. ;

Alegret, Salvador ;

Merkoci, Arben .

ANALYTICAL CHEMISTRY, 2007, 79 (14) :5232-5240

[2] Hybridization assay at a disposable electrochemical biosensor for the attomole detection of amplified human cytomegalovirus DNA [J].

Azek, F ;

Grossiord, C ;

Joannes, M ;

Limoges, B ;

Brossier, P .

ANALYTICAL BIOCHEMISTRY, 2000, 284 (01) :107-113

[3] Reactive electrophoretic activation of a microelectrode for enzyme-amplified recognition and for melting temperature determination of 105 copies of a simple oligonucleotide [J].

de Lumley-Woodyear, T ;

Caruana, DJ ;

Campbell, CN ;

Heller, A .

ANALYTICAL CHEMISTRY, 1999, 71 (02) :394-398

[4] Electrochemical DNA sensors [J].

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2003, 21 (10) :1192-1199

[5] Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA [J].

Fan, CH ;

Plaxco, KW ;

Heeger, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9134-9137

[6] Electrochemical control of a DNA Holliday junction nanoswitch by Mg2+ ions [J].

Ferapontova, E. E. ;

Mountford, C. P. ;

Crain, J. ;

Buck, A. H. ;

Dickinson, P. ;

Beattie, J. S. ;

Ghazal, P. ;

Terry, J. G. ;

Walton, A. J. ;

Mount, A. R. .

BIOSENSORS & BIOELECTRONICS, 2008, 24 (03) :422-428

[7] Electrochemical deposition of Zn on TiN microelectrode arrays for microanodes [J].

Ferapontova, E. E. ;

Terry, J. G. ;

Walton, A. J. ;

Mountford, C. P. ;

Crain, J. ;

Buck, A. H. ;

Dickinson, P. ;

Campbell, C. J. ;

Beattie, J. S. ;

Ghazal, P. ;

Mount, A. R. .

ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (02) :303-309

[8] Femtomolar electrochemical detection of DNA targets using metal sulfide nanoparticles [J].

Hansen, JA ;

Mukhopadhyay, R ;

Hansen, JO ;

Gothelf, KV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (12) :3860-3861

[9] Electroanalytical and bioelectroanalytical systems based on metal and semiconductor nanoparticles [J].

Katz, E ;

Willner, I ;

Wang, J .

ELECTROANALYSIS, 2004, 16 (1-2) :19-44

[10] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].

LAVIRON, E .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28

← 1 2 3 →