Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA

被引:34
作者
Ferapontova, Elena E. [1 ,2 ]
Hansen, Majken N. [1 ,2 ]
Saunders, Aaron M. [3 ]
Shipovskov, Stepan [2 ]
Sutherland, Duncan S. [2 ]
Gothelf, Kurt V. [1 ,2 ]
机构
[1] Aarhus Univ, Dept Chem, Ctr DNA Nanotechnol, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, INANO, DK-8000 Aarhus C, Denmark
[3] Danish Technol Inst, DK-8000 Aarhus C, Denmark
基金
新加坡国家研究基金会;
关键词
HYBRIDIZATION; MICROELECTRODE; BIOSENSORS; METAL;
D O I
10.1039/b924627d
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.
引用
收藏
页码:1836 / 1838
页数:3
相关论文
共 26 条
[11]   Pathogen detection:: A perspective of traditional methods and biosensors [J].
Lazcka, Olivier ;
Del Campo, F. Javier ;
Munoz, F. Xavier .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1205-1217
[12]   HYBRIDIZATION OF NUCLEIC-ACIDS IMMOBILIZED ON A QUARTZ CRYSTAL MICROBALANCE [J].
OKAHATA, Y ;
MATSUNOBU, Y ;
IJIRO, K ;
MUKAE, M ;
MURAKAMI, A ;
MAKINO, K .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (21) :8299-8300
[13]   Nucleic acid biosensors for environmental pollution monitoring [J].
Palchetti, Ilaria ;
Mascini, Marco .
ANALYST, 2008, 133 (07) :846-854
[14]  
Park SJ, 2002, SCIENCE, V295, P1503, DOI 10.1126/science.1067003
[15]   Precipitation of an insoluble product on enzyme monolayer electrodes for biosensor applications: Characterization by faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance analyses [J].
Patolsky, F ;
Zayats, M ;
Katz, E ;
Willner, I .
ANALYTICAL CHEMISTRY, 1999, 71 (15) :3171-3180
[16]   Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA [J].
Patolsky, F ;
Weizmann, Y ;
Willner, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (05) :770-772
[17]   Detection of single-base DNA mutations by enzyme-amplified electronic transduction [J].
Patolsky, F ;
Lichtenstein, A ;
Willner, I .
NATURE BIOTECHNOLOGY, 2001, 19 (03) :253-257
[18]   Observation of hybridization and dehybridization of thiol-tethered DNA using two-color surface plasmon resonance spectroscopy [J].
Peterlinz, KA ;
Georgiadis, RM ;
Herne, TM ;
Tarlov, MJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (14) :3401-3402
[19]   Development of biosensors for cancer clinical testing [J].
Rasooly, A ;
Jacobson, J .
BIOSENSORS & BIOELECTRONICS, 2006, 21 (10) :1851-1858
[20]  
Schmid RD, 1998, ANGEW CHEM INT EDIT, V37, P1609