Dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can initiate antigen-specific humoral immunity independent of CD4+ T cells

被引：68

作者：

Kikuchi, T

Worgall, S

Singh, R

Moore, MAS

Crystal, RG ^{[1
]}

机构：

[1] Cornell Univ, Weill Med Coll, Dept Med, Div Pulm & Crit Care Med, New York, NY 10021 USA

[2] Cornell Univ, Weill Med Coll, Dept Pediat, New York, NY USA

[3] Cornell Univ, Weill Med Coll, Belfer Gene Therapy Core Facil, New York, NY USA

[4] Cornell Univ, Weill Med Coll, Inst Med Genet, New York, NY USA

[5] Mem Sloan Kettering Canc Ctr, James Ewing Lab Dev Hematopoiesis, New York, NY 10021 USA

来源：

NATURE MEDICINE | 2000年 / 6卷 / 10期

关键词：

D O I：

10.1038/80498

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated whether dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can trigger B cells to produce antigen-specific antibodies without CD4(+) T-cell help. Dendritic cells modified with a recombinant adenovirus vector to express CD40 ligand and pulsed with heat-killed Pseudomonas induced naive B cells to produce antibodies against Pseudomonas in the absence of CD4(+) T cells in vitro, initiated Pseudomonas-specific humoral immune responses in vivo in wild-type and CD4(-/-) mice, and protected immunized wild-type and CD4(-/-), but not B-cell(-/-) mice, from lethal intrapulmonary challenge with Pseudomonas. Thus, genetic modification of dendritic cells with CD40 ligand enables them to present a complex mixture of microbial antigens and establish CD4(+) T cell-independent, B cell-mediated protective immunity against a specific microbe.

引用

页码：1154 / 1159

页数：6

共 37 条

[11] Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes [J].