Effects of the Surface Characteristics of Polyhydroxyalkanoates on the Metabolic Activities and Morphology of Human Mesenchymal Stem Cells

被引：19

作者：

Yu, Bo-Yi ^{[1
,2
]}

Chen, Po-Ya ^{[1
]}

Sun, Yi-Ming ^{[1
,3
,4
]}

Lee, Yu-Tsang ^{[1
,2
,5
]}

Young, Tai-Horng ^{[2
]}

机构：

[1] Yuan Ze Univ, Dept Chem Engn & Mat Sci, Chungli 320, Taiwan

[2] Natl Taiwan Univ, Inst Med Engn, Taipei 100, Taiwan

[3] Yuan Ze Univ, Grad Sch Biotechnol & Bioengn, Chungli 320, Taiwan

[4] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Chungli 320, Taiwan

[5] Min Sheng Gen Hosp, Dept Orthoped Surg, Tao Yuan 330, Taiwan

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2010年 / 21卷 / 01期

关键词：

Polyhydroxyalkanoates (PHAs); human mesenchymal stem cells (hMSCs); hyaluronic acid (HA); biomaterials; MARROW STROMAL CELLS; HYALURONIC-ACID; MELANOCYTE SPHEROIDS; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); DIFFERENTIATION; GROWTH; CRYSTALLIZATION; FIBROBLAST; MEMBRANES; SURVIVAL;

D O I：

10.1163/156856209X410139

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Polyhydroxyalkanoates (PHAs) are a newer family of biomaterials for tissue-engineering applications. The objective of this study is to investigate the behavior of human mesenchymal stem cells (hMSCs) grown on various PHA films. The surface characteristics of PHA co-polymer films were varied by the content of 3-hydroxyvalerate (HV) or 3-hydroxyhexanoate (HHx) and by the film preparation methods such as compression-molding and solvent-casting. Hyaluronic acid (HA) was further applied to modify the surface properties of PHA membranes. As HV content increased, the crystallinity and the hydrophobicity of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membranes decreased and the metabolic activity of hMSCs raised, although the distribution and morphology of hMSCs did not show significant variation. Hyaluronic acid (HA) coating on PHA membranes could improve the metabolic activity and reduce the death rate of hMSCs. Aggregates and spheroidal clusters of hMSCs were found on the surface of cast poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) membranes. The growth of hMSCs was remarkably influenced by various surface characteristics of the PHA films. (c) Koninklijke Brill NV, Leiden, 2010

引用

页码：17 / 36

页数：20

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