High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy

被引：266

作者：

Verveer, Peter J.

Swoger, Jim .

Pampaloni, Francesco

Greger, Klaus

Marcello, Marco

Stelzer, Ernst H. K.

机构：

[1] German Canc Res Ctr, Biomed Struct Grp, D-69120 Heidelberg, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

[3] Ctr Genom Regulat, Syst Biol Programme, E-08003 Barcelona, Spain

来源：

NATURE METHODS | 2007年 / 4卷 / 04期

关键词：

D O I：

10.1038/NMETH1017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that single ( or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.

引用

收藏

页码：311 / 313

页数：3

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