Aβ fibrillogenesis:: Kinetic parameters for fibril formation from Congo red binding

被引：45

作者：

Inouye, H ^{[1
]}

Kirschner, DA ^{[1
]}

机构：

[1] Boston Coll, Dept Biol, Chestnut Hill, MA 02467 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2000年 / 130卷 / 2-3期

关键词：

Alzheimer's beta-amyloid; fibril formation; kinetics; Congo red; nucleation-dependent self-assembly; Scatchard analysis;

D O I：

10.1006/jsbi.2000.4258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using Scatchard analysis, we have formulated as a function of time and pH the relationship between the binding of Congo red to Alzheimer's beta-amyloid and the aggregation number (i.e., monomer concentration within fibrils) as defined by nucleation-dependent self-assembly. This provides a basis on which to determine the kinetic parameters for fibril formation from the observed concentration of bound Congo red. (C) 2000 Academic Press.

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页码：123 / 129

页数：7

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