Tetrameric NAD-dependent alcohol dehydrogenase

被引：46

作者：

Karlsson, A

El-Ahmad, M

Johansson, K

Shafqat, J

Jörnvall, H

Eklund, H ^{[1
]}

Ramaswamy, S

机构：

[1] Swedish Univ Agr Sci, Dept Biol Mol, S-75124 Uppsala, Sweden

[2] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[3] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2003年 / 143卷

关键词：

zinc coordination; quaternary structure; x-ray crystallography;

D O I：

10.1016/S0009-2797(02)00222-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three-dimensional structures of the ethanol-induced, tetrameric alcohol dehydrogenase from Escherichia coli have recently been determined in the absence and presence of NAD. The structure of the E coli enzyme is similar to those of the dimeric mammalian alcohol dehydrogenases, but it has a deletion of 21 residues located at the surface of the catalytic domain. The catalytic zinc ions have two different types of coordination, which are also observed in the class III dimeric mammalian alcohol dehydrogenase. Comparison of the structures provide new insights into the relationship between tetrameric and dimeric alcohol dehydrogenases and provide a link to the structure of the tetrameric yeast alcohol dehydrogenase. circle dot 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：239 / 245

页数：7

共 27 条

[21] The coordination of the catalytic zinc ion in alcohol dehydrogenase studied by combined quantum-chemical and molecular mechanics calculations [J].