Three-color single-molecule fluorescence resonance energy transfer

被引：82

作者：

Clamme, JP ^{[1
]}

Deniz, AA ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Biol Mol, La Jolla, CA 92037 USA

来源：

CHEMPHYSCHEM | 2005年 / 6卷 / 01期

关键词：

DNA structures; fluorescence; FRET (fluorescence resonance energy transfer); single-molecule studies;

D O I：

10.1002/cphc.200400261

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A molecular ruler: A three-color single-molecule fluorescence resonance energy transfer (FRET) method is presented (see Figure inset), which can be used to simultaneously measure multiple molecular distance changes during molecular conformational changes and binding. The ability to directly study conformational subpopulations in a mixture of molecules with different interdye distances is highlighted by the well-separated peaks in the two-dimensional histogram in the Figure.

引用

页码：74 / 77

页数：4

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