Protection of chronic renal failure by a polysaccharide from Cordyceps sinensis

被引：130

作者：

Wang, Ying ^{[1
]}

Yin, Hongping ^{[1
]}

Lv, Xiaobo ^{[2
]}

Wang, Yufeng ^{[1
]}

Gao, Hongyan ^{[1
]}

Wang, Min ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210046, Peoples R China

来源：

FITOTERAPIA | 2010年 / 81卷 / 05期

关键词：

Cordyceps sinensis polysaccharide; Structure characterization; Renal injury; DEGRADATION; MEDICINE; INJURY;

D O I：

10.1016/j.fitote.2009.11.008

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

A water-soluble polysaccharide (CPS-2), isolated from the cultured Cordyceps sinensis, was obtained by hot-water extraction, anion-exchange and gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, periodate oxidation, methylation analysis, FTIR and NMR spectroscopy. CPS-2 was found to be mostly of alpha-(1 -> 4)-D-glucose and alpha-(1 -> 3)-D-mannose, branched with alpha-(1 -> 4,6)-D-glucose every twelve residues on average. CPS-2 had a molecular weight of 4.39 x 10(4) Da. The protective effect of CPS-2 on the model of chronic renal failure was established by fulgerizing kidney. The changes in blood urea nitrogen and serum creatinine revealed that CPS-2 could significantly relieve renal failure caused by fulgerizing kidney. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：397 / 402

页数：6

共 23 条

[1]

ASPINALL GO, 1957, SPECTROPHOTOMERTIC M

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].