Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside)

被引：113

作者：

Kobayashi, S ^{[1
]}

Ding, CK

Nakamura, Y

Nakajima, I

Matsumoto, R

机构：

[1] Natl Inst Fruit Tree Sci, Persimmon & Grape Res Ctr, Akitsu, Hiroshima 7292494, Japan

[2] Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

[3] Natl Inst Fruit Tree Sci, Dept Pomol, Tsukuba, Ibaraki 3058605, Japan

[4] Natl Inst Fruit Tree Sci, Dept Citriculture, Nagasaki 8592501, Japan

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 09期

关键词：

kiwifruit; stilbene synthase; transformant; resveratrol; piceid;

D O I：

10.1007/s002990000203

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A stilbene synthase gene was isolated from three Vitis spp. (V. vinifera. V. labrusca and V. riparia). These genes were placed under the control of the cauliflower mosaic virus 35S promoter and introduced into kiwifruit (Actinidia deliciosa) plants by; Agrobacterium-mediated gene transfer. The introduced gene(s) were expressed and piceid (resveratrolglucoside) rather than resveratrol was produced in the Leaves of the transformants. Resveratrol produced by the action of the integrated gene(s) seems to he metabolized into piceid by an endogenous glycosyl transferase. Among the transformants obtained, the highest piceid content in the young leaves was 182 mu g/g fresh weight. Although these transformants did not show resistance against Botrytis cinerea, which causes gray-mould disease, the fruits may have some beneficial effects on human health.

引用

页码：904 / 910

页数：7

共 23 条

[11] PRODUCTION OF RESVERATROL BY VITIS-VINIFERA AND OTHER MEMBERS OF VITACEAE AS A RESPONSE TO INFECTION OR INJURY [J].

LANGCAKE, P ;

PRYCE, RJ .

PHYSIOLOGICAL PLANT PATHOLOGY, 1976, 9 (01) :77-86

[12]

LANGCAKE P, 1979, VITIS, V18, P244

[13] Transformation and expression of a stilbene synthase gene of Vitis vinifera L. in barley and wheat for increased fungal resistance [J].

Leckband, G ;

Lörz, H .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1004-1012

[14]

MATSUTA N, 1993, TECHNIQUES GENE DIAG, P184

[15] COORDINATE-DEPENDENT AND ELICITOR-DEPENDENT EXPRESSION OF STILBENE SYNTHASE AND PHENYLALANINE AMMONIA-LYASE GENES IN VITIS CV OPTIMA [J].

MELCHIOR, F ;

KINDL, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (02) :552-557

[16] GRAPEVINE STILBENE SYNTHASE CDNA ONLY SLIGHTLY DIFFERING FROM CHALCONE SYNTHASE CDNA IS EXPRESSED IN ESCHERICHIA-COLI INTO A CATALYTICALLY ACTIVE ENZYME [J].

MELCHIOR, F ;

KINDL, H .

FEBS LETTERS, 1990, 268 (01) :17-20

[17] Expression of soybean β-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants [J].

Nakamura, Y ;

Sawada, H ;

Kobayashi, S ;

Nakajima, I ;

Yoshikawa, M .

PLANT CELL REPORTS, 1999, 18 (7-8) :527-532

[18] THE RED WINE PHENOLICS TRANS-RESVERATROL AND QUERCETIN BLOCK HUMAN PLATELET-AGGREGATION AND EICOSANOID SYNTHESIS - IMPLICATIONS FOR PROTECTION AGAINST CORONARY HEART-DISEASE [J].

PACEASCIAK, CR ;

HAHN, S ;

DIAMANDIS, EP ;

SOLEAS, G ;

GOLDBERG, DM .

CLINICA CHIMICA ACTA, 1995, 235 (02) :207-219

[19]

Sambrook J., 2002, MOL CLONING LAB MANU

[20]

SHAN CW, 1990, ACTA PHARM SINIC, V11, P527

← 1 2 3 →