Pseudomonas brassicacearum strain Am3 containing 1-aminocyclopropane-1-carboxylate deaminase can show both pathogenic and growth-promoting properties in its interaction with tomato

被引:76
作者
Belimov, A. A.
Dodd, I. C.
Safronova, V. I.
Hontzeas, N.
Davies, W. J.
机构
[1] All Russia Res Inst Agr Microbiol, St Petersburg 196608, Russia
[2] Univ Lancaster, Dept Biol Sci, Lancaster LA1 4YQ, England
[3] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada
基金
俄罗斯基础研究基金会;
关键词
ACC deaminase; colonization; ethylene; GFP; PGPR; phytopathogen; plant-bacteria interactions; Pseudomonas; rhizosphere; tomato;
D O I
10.1093/jxb/erm010
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of bacterial 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity in the interaction between tomato (Lycopersicon esculentum=Solanum lycopersicum) and Pseudomonas brassicacearum was studied in different strains. The phytopathogenic strain 520-1 possesses ACC deaminase activity, an important trait of plant growth-promoting rhizobacteria (PGPR) that stimulates root growth. The ACC-utilizing PGPR strain Am3 increased in vitro root elongation and root biomass of soil-grown tomato cv. Ailsa Craig at low bacterial concentrations (10(6) cells ml(-1) in vitro and 10(6) cells g(-1) soil) but had negative effects on in vitro root elongation at higher bacterial concentrations. A mutant strain of Am3 (designated T8-1) that was engineered to be ACC deaminase deficient failed to promote tomato root growth in vitro and in soil. Although strains T8-1 and 520-1 inhibited root growth in vitro at higher bacterial concentrations (> 10(6) cells ml(-1)), they did not cause disease symptoms in vitro after seed inoculation, or in soil supplemented with bacteria. All the P. brassicacearum strains studied caused pith necrosis when stems or fruits were inoculated with a bacterial suspension, as did the causal organism of this disease (P. corrugata 176), but the non-pathogenic strain Pseudomonas sp. Dp2 did not. Strains Am3 and T8-1 were marked with antibiotic resistance and fluorescence to show that bacteria introduced to the nutrient solution or on seeds in vitro, or in soil were capable of colonizing the root surface, but were not detected inside root tissues. Both strains showed similar colonization ability either on root surfaces or in wounded stems. The results suggest that bacterial ACC deaminase of P. brassicacearum Am3 can promote growth in tomato by masking the phytopathogenic properties of this bacterium.
引用
收藏
页码:1485 / 1495
页数:11
相关论文
共 39 条
[11]  
DUTTA S, 1991, PHYSIOL PLANTARUM, V82, P225, DOI 10.1111/j.1399-3054.1991.tb00085.x
[12]  
FEYS BJF, 1994, PLANT CELL, V6, P751, DOI 10.1105/tpc.6.5.751
[13]   A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria [J].
Glick, BR ;
Penrose, DM ;
Li, JP .
JOURNAL OF THEORETICAL BIOLOGY, 1998, 190 (01) :63-68
[14]   A NOVEL PROCEDURE FOR RAPID ISOLATION OF PLANT-GROWTH PROMOTING PSEUDOMONADS [J].
GLICK, BR ;
KARATUROVIC, DM ;
NEWELL, PC .
CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 (06) :533-536
[15]   Early development of canola seedlings in the presence of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 [J].
Glick, BR ;
Liu, CP ;
Ghosh, S ;
Dumbroff, EB .
SOIL BIOLOGY & BIOCHEMISTRY, 1997, 29 (08) :1233-1239
[16]   Root elongation in various agronomic crops by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 [J].
Hall, JA ;
Peirson, D ;
Ghosh, S ;
Glick, BR .
ISRAEL JOURNAL OF PLANT SCIENCES, 1996, 44 (01) :37-42
[17]   METABOLISM OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID [J].
HONMA, M ;
SHIMOMURA, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1978, 42 (10) :1825-1831
[18]   Evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes [J].
Hontzeas, N ;
Richardson, AO ;
Belimov, A ;
Safronova, V ;
Abu-Omar, MM ;
Glick, BR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :7556-7558
[19]   Changes in gene expression in canola roots induced by ACC-deaminase-containing plant-growth-promoting bacteria [J].
Hontzeas, N ;
Saleh, SS ;
Glick, BR .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (08) :865-871
[20]   THE STIMULATION OF ETHYLENE SYNTHESIS IN NICOTIANA-TABACUM LEAVES BY THE PHYTOTOXIN CORONATINE [J].
KENYON, JS ;
TURNER, JG .
PLANT PHYSIOLOGY, 1992, 100 (01) :219-224