An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain

被引：63

作者：

Clarke, Gerard ^{[1
]}

Mahony, Siobhain

Malone, Gwen

Dinan, Timothy G.

机构：

[1] Univ Coll Cork, Alimentary Pharmabiot Ctr, Cork, Ireland

[2] Univ Coll Cork, Dept Psychiat, Cork, Ireland

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2007年 / 160卷 / 02期

关键词：

GABA; glutamate; high performance liquid chromatography; naphthalene-2,3-dicarboxaldehyde; isocratic protocol; maternal deprivation;

D O I：

10.1016/j.jneumeth.2006.09.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The amino acid neurotransmitters gamma-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：223 / 230

页数：8

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