Binding of coatomer by the PEX11 C-terminus is not required for function

被引:18
作者
Maier, AG
Schulreich, S
Bremser, M
Clayton, C
机构
[1] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany
[2] Heidelberg Univ, Zentrum Biochem, D-69120 Heidelberg, Germany
关键词
trypanosome; microbody; glycosome; coatomer; PEX11;
D O I
10.1016/S0014-5793(00)02132-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microbodies are single membrane-bound organelles found in eukaryotes from trypanosomes to man. Although they have diverse roles in metabolism, the mechanisms and molecules involved in membrane biogenesis and matrix protein import are conserved, Similarly, the basic mechanisms and structures involved in vesicular transport are similar throughout eukaryotic evolution. The PEX11 proteins are required for the division of microbodies in trypanosomes, yeast and mammals, and a role of coatomer in this process has been suggested. We show here that the binding of trypanosome, yeast and bovine coatomers to selected peptides is identical. Coatomer binds to the C-termini of trypanosome PEX11 and rat Pex11 alpha but not yeast Pex11p or human Pex11 beta, Mutations of the C-terminus of trypanosome PEX11 that eliminated coatomer binding did not affect function in yeast or trypanosomes. Thus binding of coatomer to the C-terminus of PEX11 is not required for PEX11 function. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:82 / 86
页数:5
相关论文
共 20 条
[11]   Peroxisome biogenesis: Back to the endoplasmic reticulum? [J].
Kunau, WH ;
Erdmann, R .
CURRENT BIOLOGY, 1998, 8 (09) :R299-R302
[12]   Peroxisome targeting signal type 1 (PTS1) receptor is involved in import of both PTS1 and PTS2:: Studies with PEX5-defective CHO cell mutants [J].
Otera, H ;
Okumoto, K ;
Tateishi, K ;
Ikoma, Y ;
Matsuda, E ;
Nishimura, M ;
Tsukamoto, T ;
Osumi, T ;
Ohashi, K ;
Higuchi, O ;
Fujiki, Y .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (01) :388-399
[13]   Peroxisome biogenesis: Involvement of ARF and coatomer [J].
Passreiter, M ;
Anton, M ;
Lay, D ;
Frank, R ;
Harter, C ;
Wieland, FT ;
Gorgas, K ;
Just, WW .
JOURNAL OF CELL BIOLOGY, 1998, 141 (02) :373-383
[14]   Reversible dissociation of coatomer:: Functional characterization of a β/δ-coat protein subcomplex [J].
Pavel, J ;
Harter, C ;
Weiland, FT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2140-2145
[15]   Expression of PEX11β mediates peroxisome proliferation in the absence of extracellular stimuli [J].
Schrader, M ;
Reuber, BE ;
Morrell, JC ;
Jimenez-Sanchez, G ;
Obie, C ;
Stroh, TA ;
Valle, D ;
Schroer, TA ;
Gould, SJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (45) :29607-29614
[16]  
SOH K, 1996, J CELL BIOL, V135, P1239
[17]   Peroxisome synthesis in the absence of preexisting peroxisomes [J].
South, ST ;
Gould, SJ .
JOURNAL OF CELL BIOLOGY, 1999, 144 (02) :255-266
[18]   Components involved in peroxisome import, biogenesis, proliferation, turnover, and movement [J].
Subramani, S .
PHYSIOLOGICAL REVIEWS, 1998, 78 (01) :171-188
[19]   The endoplasmic reticulum plays an essential role in peroxisome biogenesis [J].
Titorenko, VI ;
Rachubinski, RA .
TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (07) :231-233
[20]   Mechanisms of vesicle formation: insights from the COP system [J].
Wieland, F ;
Harter, C .
CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (04) :440-446