An esterification method for determination of lipase activity

被引：70

作者：

Kiran, KR ^{[1
]}

Krishna, SH ^{[1
]}

Babu, CVS ^{[1
]}

Karanth, NG ^{[1
]}

Divakar, S ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Fermentat Technol & Bioengn Dept, Mysore 570013, Karnataka, India

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 19期

关键词：

butyl butyrate; butyric acid; esterification; lipase;

D O I：

10.1023/A:1005604206258

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The choice of a lipase for an esterification reaction can be determined from the esterification reaction between butyric acid (0.16 M) and butanol (0.33 M) at 50 degrees C and agitated with 10 mg lipase. The decrease in butyric acid is measured by titration and 1 unit of lipase activity is defined as 1 mu mol butyric acid consumed per min per mg lipase.

引用

页码：1511 / 1514

页数：4

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